Abstract
An improved micromethod for plasma fibrinogen, based on the Ellis-Stransky thrombin-clot density procedure, is presented. Modifications includes: (1) addition of Polybrene eliminates heparin interference; (2) measurement of absorbance at 340 nm provides improved sensitivity at low fibrinogen levels as well as with small volumes of plasma (40 mul.); (3) higher concentration of thrombin reduces the incubation time. Plasma fibrinogen by the proposed method compares well with a reference procedure based on total clottable protein (coefficient of correlation 0.99).
MeSH terms
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Bilirubin / blood
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Circadian Rhythm
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Disseminated Intravascular Coagulation / blood
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Disseminated Intravascular Coagulation / drug therapy
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Fibrinogen / analysis*
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Hemolysis
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Heparin Antagonists / administration & dosage
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Heparin* / therapeutic use
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Humans
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Hydrocarbons, Brominated / administration & dosage
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Lipids / blood
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Microchemistry
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Nephelometry and Turbidimetry / methods*
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Photometry / methods*
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Polyamines / administration & dosage
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Polymers / administration & dosage
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Thrombin
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Time Factors
Substances
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Heparin Antagonists
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Hydrocarbons, Brominated
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Lipids
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Polyamines
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Polymers
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Fibrinogen
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Heparin
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Thrombin
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Bilirubin