Typical CBFbeta/MYH11 fusion due to insertion of the 3'-MYH11 gene into 16q22 in acute monocytic leukemia with normal chromosomes 16 and trisomies 8 and 22

Cancer Genet Cytogenet. 2000 Dec;123(2):137-9. doi: 10.1016/s0165-4608(00)00321-6.


In a case of acute monocytic leukemia, M5a according to the FAB classification, with a 48,XY,+8,+22 karyotype, amplification of the CBFbeta/MYH11 fusion transcript type A was detected by reverse transcriptase-polymerase chain reaction (RT-PCR). Fluorescence in situ hybridization (FISH) using an appropriate panel of DNA probes showed that insertion of the 3'-MYH11 within the CBFbeta gene on chromosome 16q22 was the mechanism producing the same molecular rearrangement as in typical inv(16)(p13q22)/t(16;16)(p13;q22).

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Chromosomes, Human, Pair 16 / genetics*
  • Chromosomes, Human, Pair 22 / genetics*
  • Chromosomes, Human, Pair 8 / genetics*
  • Humans
  • In Situ Hybridization, Fluorescence
  • Leukemia, Monocytic, Acute / genetics*
  • Leukemia, Monocytic, Acute / pathology
  • Male
  • Oncogene Proteins, Fusion / genetics*
  • Trisomy*


  • CBFbeta-MYH11 fusion protein
  • Oncogene Proteins, Fusion