Integrin-associated protein (CD47/IAP) contributes to T cell arrest on inflammatory vascular endothelium under flow

FASEB J. 2001 Feb;15(2):341-50. doi: 10.1096/fj.99-0833com.


Integrin-associated protein (CD47/IAP) is a pentaspan molecule that regulates integrin functions. We prepared a CD47-deficient Jurkat T cell line to assess its role in the arrest of T cells on inflammatory endothelium. Under flow conditions, constitutive arrest of CD47-deficient cells is strongly decreased as compared to the original cell line, whereas reexpression of CD47 reestablishes their ability to stop. Moreover, cells transfected with a chimera made with the extracellular portion of CD47 and the transmembrane domain of CD7 or several truncated forms of CD47 show that the first transmembrane domain and a short cytoplasmic loop are sufficient for this process. CD47 effect is indirect and depends mainly on the alpha4beta1/VCAM-1 pathway, as shown by blocking antibodies. We detected on endothelium the two CD47 counter receptors known to date: thrombospondin and SIRP1alpha. Blocking experiments show that both are involved. Overall, CD47 participates in the constitutive arrest of T lymphocytes on inflamed vascular endothelium by up-regulating alpha 4beta1 integrins.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antibodies, Monoclonal / pharmacology
  • Antigens, CD / genetics
  • Antigens, CD / physiology*
  • CD4-Positive T-Lymphocytes / immunology
  • CD47 Antigen
  • Carrier Proteins / genetics
  • Carrier Proteins / physiology*
  • Cells, Cultured
  • Endothelium, Vascular / immunology
  • Endothelium, Vascular / physiology*
  • Humans
  • Inflammation
  • Integrin alpha4beta1
  • Integrins / immunology
  • Integrins / physiology
  • Jurkat Cells
  • Mutagenesis
  • Receptors, Lymphocyte Homing / immunology
  • Receptors, Lymphocyte Homing / physiology
  • Recombinant Fusion Proteins / metabolism
  • Recombinant Proteins / pharmacology
  • Sequence Deletion
  • Signal Transduction
  • Stress, Mechanical
  • T-Lymphocytes / immunology*
  • Transfection
  • Tumor Necrosis Factor-alpha / pharmacology
  • Up-Regulation
  • Vascular Cell Adhesion Molecule-1 / immunology
  • Vascular Cell Adhesion Molecule-1 / pharmacology
  • Vascular Cell Adhesion Molecule-1 / physiology


  • Antibodies, Monoclonal
  • Antigens, CD
  • CD47 Antigen
  • CD47 protein, human
  • Carrier Proteins
  • Integrin alpha4beta1
  • Integrins
  • Receptors, Lymphocyte Homing
  • Recombinant Fusion Proteins
  • Recombinant Proteins
  • Tumor Necrosis Factor-alpha
  • Vascular Cell Adhesion Molecule-1