Abstract
To investigate iron uptake, a chromosomal locus containing three consecutive open reading frames, designated fhuC, fhuB, and fhuD, was identified in Staphylococcus aureus. Whereas the fhuC gene encodes an ATP-binding protein, fhuB and fhuD code for ferrichrome permeases and thus resemble an ATP-binding cassette transporter. A fhuB knockout mutant showed impaired uptake of iron bound to the siderophores but not of ferric chloride, suggesting that this operon is specific for siderophore-mediated iron uptake.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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ATP-Binding Cassette Transporters
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Carrier Proteins / genetics
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Carrier Proteins / metabolism
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Culture Media
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Escherichia coli Proteins*
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Ferric Compounds / metabolism*
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Hydroxamic Acids / metabolism*
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Membrane Proteins / genetics
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Membrane Proteins / metabolism
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Membrane Transport Proteins*
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Molecular Sequence Data
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Mutation
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Operon*
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Periplasmic Binding Proteins*
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Staphylococcus aureus / genetics*
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Staphylococcus aureus / metabolism*
Substances
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ATP-Binding Cassette Transporters
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Carrier Proteins
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Culture Media
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Escherichia coli Proteins
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Ferric Compounds
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FhuC protein, E coli
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Hydroxamic Acids
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Membrane Proteins
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Membrane Transport Proteins
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Periplasmic Binding Proteins
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iron (III) hydroxamate
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fhuB protein, E coli
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fhuD protein, E coli