Plasmid-mediated and inducible cephalosporinase DHA-2 from Klebsiella pneumoniae

J Antimicrob Chemother. 2001 Feb;47(2):207-10. doi: 10.1093/jac/47.2.207.


A Klebsiella pneumoniae strain resistant to cefoxitin and oxyimino-cephalosporins was cultured from a child hospitalized in Paris, France, in 1992. This isolate harboured a beta-lactamase gene located on an approximately 200 kb non-self-transferable plasmid. The beta-lactamase identified, DHA-2, shared 99% amino acid identity with the AmpC enzyme of Morganella morganii. DHA-2 was a point-mutant derivative of DHA-1 identified previously in a Salmonella enteritidis isolate. DHA-2 expression was inducible due to an ampR regulatory gene. This is the first report of an inducible and plasmid-located cephalosporinase from K. pneumoniae.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / analysis
  • Bacterial Proteins / metabolism
  • Ceftazidime / pharmacology
  • Cephalosporins / pharmacology
  • Drug Resistance, Microbial
  • Enzyme Induction / drug effects
  • Isoelectric Focusing
  • Klebsiella pneumoniae / enzymology*
  • Klebsiella pneumoniae / genetics*
  • Microbial Sensitivity Tests
  • Molecular Sequence Data
  • Phenotype
  • Plasmids / genetics*
  • Reverse Transcriptase Polymerase Chain Reaction
  • beta-Lactamases / analysis
  • beta-Lactamases / biosynthesis*
  • beta-Lactamases / genetics*
  • beta-Lactamases / metabolism


  • Bacterial Proteins
  • Cephalosporins
  • Ceftazidime
  • cephalosporinase DHA-2
  • beta-Lactamases

Associated data

  • GENBANK/AF259520