Siderophore uptake and use by the yeast Saccharomyces cerevisiae

Microbiology. 2001 Feb;147(Pt 2):289-298. doi: 10.1099/00221287-147-2-289.

Abstract

The non-reductive uptake of several siderophores (ferrioxamine B, ferrichrome, triacetylfusarinine C and ferricrocin) by various strains of Saccharomyces cerevisiae was studied. Several aspects of siderophore transport were examined, including specificity of transport, regulation of transport and intracellular localization of the ferri-siderophores. Ferrioxamine B was taken up preferentially via the products of the SIT1 gene and triacetylfusarinine C by the TAF1 gene product, but the specificity was not absolute. Ferrichrome and ferricrocin uptake was not dependent on a single major facilitator superfamily (MFS) gene product. The apparent specificity of transport was strongly dependent on the genetic background of the cells. Non-reductive uptake of siderophores was induced under more stringent conditions (of iron deprivation) than was the reductive uptake of ferric citrate. Regulation of transport depended on the transcriptional factors Aft1 and Tup1/Ssn6. Cells disrupted for the TUP1 or SSN6 genes were constitutively derepressed for the uptake of ferrichrome, ferricrocin or ferrioxamine B, but not for the uptake of triacetylfusarinine C. Cells bearing the AFT1(up) mutation accumulated large amounts of ferric siderophores. Intracellular decomplexation of the siderophores occurred when transcription of the AFT1(up) gene was repressed. Ferrioxamine B and ferrichrome seemed to accumulate in an endosomal compartment, as shown by biochemical studies and by confocal microscopy study of cells loaded with a fluorescent derivative of ferrichrome. Endocytosis was, however, not involved in the non-reductive uptake of siderophores.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism
  • DNA-Binding Proteins*
  • Ferric Compounds / metabolism
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism
  • Gene Expression Regulation, Fungal*
  • Iron / metabolism
  • Membrane Transport Proteins*
  • Nuclear Proteins*
  • Phosphoprotein Phosphatases*
  • Protein Phosphatase 2
  • Repressor Proteins*
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / growth & development
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins*
  • Siderophores / metabolism*
  • Transcription Factors / genetics
  • Transcription Factors / metabolism

Substances

  • AFT1 protein, S cerevisiae
  • ARN2 protein, S cerevisiae
  • CYC8 protein, S cerevisiae
  • Carrier Proteins
  • DNA-Binding Proteins
  • Ferric Compounds
  • Fungal Proteins
  • Membrane Transport Proteins
  • Nuclear Proteins
  • Repressor Proteins
  • Saccharomyces cerevisiae Proteins
  • Siderophores
  • TUP1 protein, S cerevisiae
  • Transcription Factors
  • Iron
  • Phosphoprotein Phosphatases
  • Protein Phosphatase 2
  • SIT4 protein, S cerevisiae