Changes in carboxysome structure and grouping and in photosynthetic affinity for inorganic carbon in Anabaena strain PCC 7119 (Cyanophyta) in response to modification of CO2 and Na+ supply

Plant Cell Physiol. 2001 Jan;42(1):46-53. doi: 10.1093/pcp/pce005.

Abstract

In ANABAENA: PCC 7119 a 4-fold decrease in the value of the apparent photosynthetic affinity for external inorganic carbon [K1/2 (Ci)] occurred between 9 and 12 h after the transfer from high-CO2 (2% CO2-enriched air) to air-growing conditions. A slight increase in carboxysome frequency occurred, but during this transition their appearance and distribution remained unchanged. ANABAENA: PCC 7119 did not improve its K1/2 (Ci) beyond the above cited level of acclimation neither by culturing the cyanobacteria in Na+-deficient medium in air nor by aeration with CO2-depleted air. In air-grown cultures, Na+ deficiency induced a large increase in carboxysome frequency and an alteration of their appearance: the greatest proportion were electron-dense whereas this type constituted a minority in high-CO2 and in air, Na+-sufficient conditions. It also induced major changes in carboxysome distribution, whereby more than 60% were grouped, compared with only 10% in high-CO2 and in air, Na+-sufficient conditions. These changes in carboxysome expression were extremely rapid, occurring mainly during the first 2 h.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anabaena / growth & development
  • Anabaena / metabolism*
  • Anabaena / ultrastructure
  • Carbon / metabolism*
  • Carbon Dioxide / pharmacology*
  • Cells, Cultured
  • Microscopy, Electron
  • Organelles / metabolism*
  • Organelles / ultrastructure
  • Photosynthesis / drug effects
  • Ribulose-Bisphosphate Carboxylase / analysis
  • Ribulose-Bisphosphate Carboxylase / metabolism
  • Sodium / pharmacology*
  • Time Factors

Substances

  • Carbon Dioxide
  • Carbon
  • Sodium
  • Ribulose-Bisphosphate Carboxylase