Requirement of Shp-2 tyrosine phosphatase in lymphoid and hematopoietic cell development

Blood. 2001 Feb 15;97(4):911-4. doi: 10.1182/blood.v97.4.911.

Abstract

Shp-1 and Shp-2 are cytoplasmic phosphotyrosine phosphatases with similar structures. Mice deficient in Shp-2 die at midgestation with defects in mesodermal patterning, and a hypomorphic mutation at the Shp-1 locus results in the moth-eaten viable (me(v)) phenotype. Previously, a critical role of Shp-2 in mediating erythroid/myeloid cell development was demonstrated. By using the RAG-2-deficient blastocyst complementation, the role of Shp-2 in lymphopoiesis has been determined. Chimeric mice generated by injecting Shp-2(-/-) embryonic stem cells into Rag-2-deficient blastocysts had no detectable mature T and B cells, serum immunoglobulin M, or even Thy-1(+) and B220(+) precursor lymphocytes. Collectively, these results suggest a positive role of Shp-2 in the development of all blood cell lineages, in contrast to the negative effect of Shp-1 in this process. To determine whether Shp-1 and Shp-2 interact in hematopoiesis, Shp-2(-/-):me(v)/me(v) double-mutant embryos were generated and the hematopoietic cell development in the yolk sacs was examined. More hematopoietic stem/progenitor cells were detected in Shp-2(-/-):me(v)/me(v) embryos than in Shp-2(-/-) littermates. The partial rescue by Shp-1 deficiency of the defective hematopoiesis caused by the Shp-2 mutation suggests that Shp-1 and Shp-2 have antagonistic effects in hematopoiesis, possibly through a bidirectional modulation of the same signaling pathway(s).

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • B-Lymphocytes / cytology
  • Cell Lineage
  • Chimera
  • DNA-Binding Proteins / deficiency
  • DNA-Binding Proteins / genetics
  • Embryonic and Fetal Development / genetics
  • Female
  • Genetic Complementation Test
  • Genotype
  • Hematopoiesis / genetics
  • Hematopoiesis / physiology*
  • Hematopoietic Stem Cells / enzymology*
  • Immunologic Deficiency Syndromes / genetics*
  • Lymphocytes / cytology
  • Lymphocytes / enzymology*
  • Mice
  • Mice, Knockout
  • Phosphorylation
  • Protein Processing, Post-Translational
  • Signal Transduction
  • Specific Pathogen-Free Organisms
  • T-Lymphocytes / cytology
  • Yolk Sac / cytology

Substances

  • DNA-Binding Proteins
  • Rag2 protein, mouse
  • V(D)J recombination activating protein 2