Identification and characterization of a novel human vanilloid receptor-like protein, VRL-2

Physiol Genomics. 2001 Jan 19;4(3):165-74. doi: 10.1152/physiolgenomics.2001.4.3.165.


Remarkable progress has been made recently in identifying a new gene family related to the capsaicin (vanilloid) receptor, VR1. Using a combination of in silico analysis of expressed sequence tag (EST) databases and conventional molecular cloning, we have isolated a novel vanilloid-like receptor, which we call VRL-2, from human kidney. The translated gene shares 46% and 43% identity with VR1 and VRL-1, respectively, and maps to chromosome 12q23-24.1, a locus associated with bipolar affective disorder. VRL-2 mRNA was most strongly expressed in the trachea, kidney, and salivary gland. An affinity-purified antibody against a peptide incorporating the COOH terminal of the receptor localized VRL-2 immunolabel in the distal tubules of the kidney, the epithelial linings of both trachea and lung airways, serous cells of submucosal glands, and mononuclear cells. Unlike VR1 and VRL-1, VRL-2 was not detected in cell bodies of dorsal root ganglia (DRG) or sensory nerve fibers. However, VRL-2 was found on sympathetic and parasympathetic nerve fibers, such as those innervating the arrector pili smooth muscle in skin, sweat glands, intestine, and blood vessels. At least four vanilloid receptor-like genes exist, the newest member, VRL-2 is found in airway and kidney epithelia and in the autonomic nervous system.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Blotting, Northern
  • Cation Transport Proteins*
  • Cell Line
  • Chromosome Mapping
  • Chromosomes, Human, Pair 12 / genetics
  • Cloning, Molecular
  • DNA, Complementary / chemistry
  • DNA, Complementary / genetics
  • DNA, Complementary / isolation & purification
  • Female
  • Humans
  • Immunohistochemistry
  • In Situ Hybridization, Fluorescence
  • Ion Channels*
  • Male
  • Molecular Sequence Data
  • RNA / genetics
  • RNA / metabolism
  • Radiation Hybrid Mapping
  • Rats
  • Receptors, Drug / genetics*
  • Receptors, Drug / metabolism
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • TRPV Cation Channels
  • Tissue Distribution


  • Cation Transport Proteins
  • DNA, Complementary
  • Ion Channels
  • Receptors, Drug
  • TRPV Cation Channels
  • TRPV4 protein, human
  • Trpv4 protein, rat
  • RNA