TRP-PLIK, a bifunctional protein with kinase and ion channel activities

Science. 2001 Feb 9;291(5506):1043-7. doi: 10.1126/science.1058519. Epub 2001 Jan 18.

Abstract

We cloned and characterized a protein kinase and ion channel, TRP-PLIK. As part of the long transient receptor potential channel subfamily implicated in control of cell division, it is a protein that is both an ion channel and a protein kinase. TRP-PLIK phosphorylated itself, displayed a wide tissue distribution, and, when expressed in CHO-K1 cells, constituted a nonselective, calcium-permeant, 105-picosiemen, steeply outwardly rectifying conductance. The zinc finger containing alpha-kinase domain was functional. Inactivation of the kinase activity by site-directed mutagenesis and the channel's dependence on intracellular adenosine triphosphate (ATP) demonstrated that the channel's kinase activity is essential for channel function.

MeSH terms

  • Adenosine Triphosphate / metabolism
  • Amino Acid Motifs
  • Amino Acid Sequence
  • Animals
  • CHO Cells
  • Calcium / metabolism
  • Catalytic Domain
  • Cations / metabolism
  • Cell Line
  • Cricetinae
  • DNA, Complementary
  • Electric Conductivity
  • Humans
  • Ion Channels / chemistry
  • Ion Channels / genetics*
  • Ion Channels / metabolism*
  • Membrane Proteins*
  • Mice
  • Molecular Sequence Data
  • Mutation
  • Myelin Basic Protein / metabolism
  • Patch-Clamp Techniques
  • Phosphorylation
  • Protein Kinases / chemistry
  • Protein Kinases / genetics*
  • Protein Kinases / metabolism*
  • Protein-Serine-Threonine Kinases
  • Rats
  • Recombinant Fusion Proteins / chemistry
  • Recombinant Fusion Proteins / metabolism
  • TRPM Cation Channels
  • Transfection
  • Two-Hybrid System Techniques
  • Type C Phospholipases / metabolism

Substances

  • Cations
  • DNA, Complementary
  • Ion Channels
  • Membrane Proteins
  • Myelin Basic Protein
  • Recombinant Fusion Proteins
  • TRPM Cation Channels
  • Adenosine Triphosphate
  • Protein Kinases
  • Trpm7 protein, mouse
  • Protein-Serine-Threonine Kinases
  • TRPM7 protein, human
  • Trpm7 protein, rat
  • Type C Phospholipases
  • Calcium