Abstract
After infection of CEM174.T2 cells [deficient for the transporter of antigen presentation (TAP)] with measles virus (MV) the nucleocapsid protein is recognized by L(d)-restricted cytotoxic T cells in a TAP-independent, chloroquine-sensitive fashion. Presentation via the TAP-independent pathway requires virus replication. During MV infection of the cell the nucleocapsid as well as the matrix protein enter the endolysosomal compartment as indicated by colocalization with the lysosomal-associated membrane protein 1 (LAMP-1). Similarly, the nucleocapsid protein of canine distemper virus (CDV) is recognized in a TAP-independent fashion. In addition, a recombinant MV expressing bacterial beta-galactosidase protein is able to introduce the recombinant antigen into the TAP-independent pathway whereas a vaccinia virus expressing beta-galactosidase is not. These data and a report about TAP-independent recognition of parainfluenza virus type 1 suggest that members of the Paramyxoviridae family regularly introduce viral proteins into the TAP-independent antigen-processing pathway.
MeSH terms
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Animals
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Antigen Presentation / immunology*
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Antigens, CD / metabolism
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Antigens, Viral / immunology
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Antigens, Viral / metabolism
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Cell Line
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Cells, Cultured
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Chloroquine / pharmacology
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Distemper Virus, Canine / genetics
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Distemper Virus, Canine / immunology*
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Endosomes / drug effects
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Endosomes / metabolism
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Epitopes / immunology
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Genetic Vectors / genetics
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Histocompatibility Antigens Class I / immunology*
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L Cells
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Lysosomal Membrane Proteins
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Lysosomes / drug effects
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Lysosomes / metabolism
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Measles virus / genetics
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Measles virus / immunology*
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Measles virus / physiology
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Membrane Glycoproteins / metabolism
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Mice
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Mice, Inbred BALB C
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Nucleocapsid / immunology
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Nucleocapsid / metabolism
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Recombinant Fusion Proteins / immunology
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Recombinant Fusion Proteins / metabolism
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T-Lymphocytes, Cytotoxic / immunology
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Vaccinia virus / genetics
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Vaccinia virus / immunology
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Viral Matrix Proteins / immunology
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Viral Matrix Proteins / metabolism
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Virus Replication
Substances
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Antigens, CD
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Antigens, Viral
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Epitopes
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Histocompatibility Antigens Class I
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Lysosomal Membrane Proteins
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Membrane Glycoproteins
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Recombinant Fusion Proteins
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Viral Matrix Proteins
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Chloroquine