Absence of rearrangements in the BRCA2 gene in human cancers

Br J Cancer. 2001 Jan;84(2):193-5. doi: 10.1054/bjoc.2000.1577.


Mutations of BRCA2 in sporadic breast and ovarian carcinomas are exceedingly rare. This led to the suggestion that large genomic rearrangements could be involved. We performed Southern blots in genomic DNA from 130 primary breast cancers and 83 cancer cell lines (breast, ovarian, pancreatic and small cell lung carcinomas) and found no genomic rearrangements. These results suggest that a gene other than BRCA2 is the target of the frequent 13q12.3 allelic deletions in human cancers.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • BRCA2 Protein
  • Blotting, Southern
  • Breast Neoplasms / genetics*
  • Breast Neoplasms / pathology
  • DNA, Neoplasm / genetics
  • Female
  • Gene Rearrangement
  • Humans
  • Neoplasm Proteins / genetics*
  • Polymorphism, Restriction Fragment Length
  • Transcription Factors / genetics*
  • Tumor Cells, Cultured


  • BRCA2 Protein
  • DNA, Neoplasm
  • Neoplasm Proteins
  • Transcription Factors