Coordination of fibroblast growth factor receptor 1 (FGFR1) and fibroblast growth factor-2 (FGF-2) trafficking to nuclei of reactive astrocytes around cerebral lesions in adult rats

Mol Cell Neurosci. 2001 Jan;17(1):17-30. doi: 10.1006/mcne.2000.0920.


Traumatic injury to the adult central nervous system initiates a cascade of cellular and trophic events, culminating in the formation of a reactive gliotic scar through which transected axons fail to regenerate. Levels of fibroblast growth factor-2 (FGF-2), a potent gliogenic and neurotrophic factor, together with its full-length receptor, FGF receptor 1 (FGFR1) are coordinately and significantly increased postinjury in both nuclear and cytoplasmic fractions of extracted cerebral cortex biopsies after a penetrant injury. FGFR1 is colocalized with FGF-2 in the nuclei of reactive astrocytes, and here FGF-2 is associated with nuclear euchromatin. This study unequivocally demonstrates coordinate up-regulation and trafficking of FGF-2 and full-length FGFR1 to the nucleus of reactive astrocytes in an in vivo model of brain injury, thereby implicating a role in nuclear activity for these molecules. However, the precise contribution of nuclear FGF-2/FGFR1 to the pathophysiological response of astrocytes after injury is undetermined.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Active Transport, Cell Nucleus / physiology*
  • Animals
  • Astrocytes / metabolism*
  • Astrocytes / pathology
  • Blotting, Western
  • Brain / metabolism
  • Brain / pathology
  • Cell Nucleus / metabolism
  • Cell Nucleus / ultrastructure
  • Disease Models, Animal
  • Euchromatin / metabolism
  • Euchromatin / ultrastructure
  • Female
  • Fibroblast Growth Factor 2 / metabolism*
  • Gliosis / etiology
  • Gliosis / metabolism
  • Gliosis / pathology
  • Head Injuries, Penetrating / complications
  • Head Injuries, Penetrating / metabolism*
  • Head Injuries, Penetrating / pathology
  • Immunohistochemistry
  • Protein Isoforms / metabolism
  • Rats
  • Rats, Wistar
  • Receptor Protein-Tyrosine Kinases / metabolism*
  • Receptor, Fibroblast Growth Factor, Type 1
  • Receptors, Fibroblast Growth Factor / metabolism*
  • Subcellular Fractions / metabolism
  • Up-Regulation


  • Euchromatin
  • Protein Isoforms
  • Receptors, Fibroblast Growth Factor
  • Fibroblast Growth Factor 2
  • Fgfr1 protein, rat
  • Receptor Protein-Tyrosine Kinases
  • Receptor, Fibroblast Growth Factor, Type 1