A new subfamily of high molecular mass CDC2-related kinases with PITAI/VRE motifs

Biochem Biophys Res Commun. 2000 Dec 29;279(3):832-7. doi: 10.1006/bbrc.2000.4042.


Kinases of the CDC2 family play a key role in cell cycle regulation and gene expression. In the present work, we identified sea urchin and human cDNAs encoding homologues of a high molecular mass CDC2-like kinase (designated CDC2L5) sharing respectively a PITAVRE and PITAIRE motif. The human cDNA encodes the full-length amino acid sequence of the cholinesterase-related cell division controller (CHED) kinase, a previously published partial coding sequence. CDC2L5 overexpressed in mammalian cells is an approximately 170-kDa nuclear protein. The mRNA is present during the sea urchin early embryogenesis and is ubiquitously expressed in human tissues.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Motifs
  • Amino Acid Sequence
  • Animals
  • CDC2-CDC28 Kinases
  • Cell Cycle / physiology
  • Cells, Cultured
  • Cloning, Molecular
  • Cyclin-Dependent Kinases / chemistry*
  • Cyclin-Dependent Kinases / genetics
  • Cyclin-Dependent Kinases / isolation & purification
  • DNA, Complementary / analysis
  • Embryo, Mammalian / physiology
  • Embryo, Nonmammalian
  • Humans
  • Molecular Sequence Data
  • Phylogeny
  • Sea Urchins / enzymology
  • Sequence Homology, Amino Acid
  • Transfection


  • DNA, Complementary
  • CDC2-CDC28 Kinases
  • Cyclin-Dependent Kinases

Associated data

  • GENBANK/AJ297709
  • GENBANK/AJ297710
  • GENBANK/AJ297711