Cloning, expression, and ontogeny of mouse organic anion-transporting polypeptide-5, a kidney-specific organic anion transporter

Biochem Biophys Res Commun. 2001 Jan 12;280(1):92-8. doi: 10.1006/bbrc.2000.4072.


The full-length coding sequence of mouse organic anion-transporting polypeptide (designated mouse Oatp-5) has been cloned from mouse kidney cDNA library. Analysis of the 5'-untranslated region (5'-UTR) of Oatp-5 cDNA through capsite cloning reveals two possible transcription start sites that are 4-bp apart. The 3'-untranslated region (3'-UTR) of Oatp-5 cDNA contains an early polyadenylation signal, indicating the possibility that mRNAs with different 3'-UTR lengths may coexist. Deduced amino acid sequence of mouse Oatp-5 protein contains 670 amino acids and has 10 putative transmembrane domains, multiple potential glycosylation and phosphorylation sites. Tissue-specific expression studies indicate that mouse Oatp-5 is expressed only in kidney. Studies on the developmental expression reveal that there is no significant expression of Oatp-5 mRNA in mouse kidney for at least 3 weeks after birth, and adult levels of Oatp-5 mRNA expression are attained more than 6 weeks after birth. Phylogenetic analysis reveals that mouse Oatp-5 is an ortholog of rat Oatp-5.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aging / physiology*
  • Amino Acid Sequence
  • Animals
  • Anion Transport Proteins
  • Base Sequence
  • Carrier Proteins / chemistry
  • Carrier Proteins / genetics*
  • Gene Expression Regulation, Developmental*
  • Humans
  • Kidney / growth & development
  • Kidney / metabolism*
  • Mice
  • Models, Molecular
  • Molecular Sequence Data
  • Organic Anion Transporters*
  • Phylogeny*
  • Protein Structure, Secondary
  • RNA, Messenger / genetics
  • Rats
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Transcription, Genetic


  • Anion Transport Proteins
  • Carrier Proteins
  • Organic Anion Transporters
  • RNA, Messenger
  • Slco1a6 protein, mouse

Associated data

  • GENBANK/AF203701
  • GENBANK/AF213260