Two isoforms of trimming glucosidase II exist in mammalian tissues and cell lines but not in yeast and insect cells

Biochem Biophys Res Commun. 2001 Jan 12;280(1):363-7. doi: 10.1006/bbrc.2000.4082.

Abstract

We previously cloned glucosidase II and provided in vivo evidence for its involvement in protein folding quality control. DNA-sequencing of different clones demonstrated the existence of two isoforms of glucosidase II which differed by 66 nucleotides due to alternative splicing. The existence of two enzyme isoforms in various organs of pig and rat as well as human, bovine, rat, and mouse cell lines could be demonstrated by RT-PCR and Western blotting. Furthermore, the two isoforms of glucosidase II could be detected in embryonic and postnatal rat kidney and liver. In yeast, Saccharomyces cerevisiae, and in insects, Drosophila S2 cells, only one isoforms of the enzyme was detectable. The ubiquitous occurrence of the two glucosidase II isoforms in mammalian tissues and cell lines might be indicative of a special function of each isoform.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cattle
  • Cell Line
  • Drosophila
  • Female
  • Gene Expression Regulation, Developmental
  • Gene Expression Regulation, Enzymologic
  • Humans
  • Isoenzymes / genetics
  • Isoenzymes / metabolism
  • Kidney / embryology
  • Kidney / enzymology*
  • Kidney / growth & development
  • Liver / embryology
  • Liver / enzymology*
  • Liver / growth & development
  • Male
  • Mammals
  • Mice
  • Organ Specificity
  • Rats
  • Reverse Transcriptase Polymerase Chain Reaction
  • Saccharomyces cerevisiae / enzymology*
  • alpha-Glucosidases / genetics*
  • alpha-Glucosidases / metabolism

Substances

  • Isoenzymes
  • 4-nitrophenyl-alpha-glucosidase
  • alpha-Glucosidases