A novel gene, CRR9, which was up-regulated in CDDP-resistant ovarian tumor cell line, was associated with apoptosis

Biochem Biophys Res Commun. 2001 Feb 2;280(4):1148-54. doi: 10.1006/bbrc.2001.4250.


In the screening for cisplatin (CDDP)-resistance related genes by a mRNA differential display method, we detected some increased bands in CDDP resistant ovarian tumor cell line 2008/C13*5.25. One of them, named DD9, was a positive fragment on Northern blot analysis. We cloned it as a full length cDNA by 5'RACE and found a novel gene, CRR9 (Cisplatin Resistance Related gene 9). The CRR9 gene was transcribed into a 2.0 kb mRNA, encoding 512 amino acids. The putative protein had transmembrane-like domains and well conserved on C terminus with human CLPTM1 and the homologs found in Drosophila and C. elegans. Transfection assay showed that the CDDP-sensitive strain 2008 with CRR9 was more sensitive to CDDP, indicating that CRR9 was not associated with the CDDP-resistance, but the CDDP-induced apoptosis.

MeSH terms

  • Amino Acid Sequence
  • Antineoplastic Agents / pharmacology*
  • Apoptosis*
  • Base Sequence
  • Blotting, Northern
  • Cisplatin / pharmacology*
  • Cloning, Molecular
  • DNA, Complementary / metabolism
  • Dose-Response Relationship, Drug
  • Drug Resistance, Neoplasm*
  • Female
  • Gene Expression Profiling
  • Humans
  • Membrane Proteins / genetics*
  • Membrane Proteins / metabolism*
  • Molecular Sequence Data
  • Neoplasm Proteins*
  • Oligonucleotides, Antisense
  • Ovarian Neoplasms / metabolism*
  • Ovarian Neoplasms / pathology*
  • Protein Structure, Tertiary
  • Proto-Oncogene Proteins c-bcl-2 / metabolism
  • RNA, Messenger / metabolism
  • Sequence Homology, Amino Acid
  • Transcription, Genetic
  • Transfection
  • Tumor Cells, Cultured
  • Up-Regulation*


  • Antineoplastic Agents
  • CLPTM1L protein, human
  • DNA, Complementary
  • Membrane Proteins
  • Neoplasm Proteins
  • Oligonucleotides, Antisense
  • Proto-Oncogene Proteins c-bcl-2
  • RNA, Messenger
  • Cisplatin