Upregulation of HSP47 and collagen type III in the dermal fibrotic disease, keloid

Biochem Biophys Res Commun. 2001 Feb 9;280(5):1316-22. doi: 10.1006/bbrc.2001.4257.

Abstract

Keloid is a dermal fibrotic disease characterized by excessive accumulation of mainly type I collagen in extracellular matrix of the dermis. We have studied the expression levels of collagen types I and III, and its molecular chaperone HSP47 in keloid lesions and surrounding unaffected skin using Northern and Western blotting and immunohistochemical analyses. Collagen types I and III mRNA levels were found to be upregulated 20-fold in keloid tissues, contradicting previous reports of nearly normal type III collagen levels in this disease. HSP47 expression in keloid lesions was also highly upregulated; eightfold at mRNA level and more than 16-fold at the protein level. Strong upregulation of these three proteins in keloid was confirmed by immunohistochemical staining. These results suggest that accumulation of both type I and type III collagen is important for the development of keloid lesions, and that HSP47 plays a role in the rapid and extensive synthesis of collagen in keloid tissues.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Collagen / analysis
  • Collagen / genetics*
  • Female
  • Gene Expression Regulation
  • HSP47 Heat-Shock Proteins
  • Heat-Shock Proteins / analysis
  • Heat-Shock Proteins / genetics*
  • Humans
  • Immunohistochemistry
  • Keloid / genetics*
  • Keloid / metabolism
  • Keloid / pathology
  • Male
  • Middle Aged
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Up-Regulation

Substances

  • HSP47 Heat-Shock Proteins
  • Heat-Shock Proteins
  • RNA, Messenger
  • SERPINH1 protein, human
  • Collagen