Chemopreventive agent resveratrol, a natural product derived from grapes, reversibly inhibits progression through S and G2 phases of the cell cycle in U937 cells

Cancer Lett. 2001 Feb 10;163(1):43-9. doi: 10.1016/s0304-3835(00)00658-3.

Abstract

Resveratrol, a natural product derived from grapes, has been shown to prevent carcinogenesis in murine models. We report here that resveratrol induces antiproliferation and arrests the S phase in human histiocytic lymphoma U937 cells. Resveratrol induces arrest in the S phase at low concentrations (30-60 microM), but high concentrations do not induce S phase accumulation in U937 cells. Removal of resveratrol from the culture medium stimulates U937 cells to reenter the cell cycle synchronously, as judged by the expression patterns of cyclin E, A and by fluorescent activated cell sorting analysis. These data demonstrate that resveratrol causes S phase arrest and reversible cell cycle arrest. Thus, resveratrol provides an important new cell cycle blocker as well as a cancer chemopreventive agent.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents, Phytogenic / pharmacology*
  • Blotting, Western
  • Cell Count
  • Cell Cycle / drug effects
  • Cell Cycle Proteins / drug effects
  • Cell Cycle Proteins / metabolism
  • Cell Division / drug effects
  • Dose-Response Relationship, Drug
  • Flow Cytometry
  • G2 Phase / drug effects*
  • Humans
  • Resveratrol
  • Rosales / chemistry*
  • S Phase / drug effects*
  • Stilbenes / pharmacology*
  • Time Factors
  • U937 Cells

Substances

  • Antineoplastic Agents, Phytogenic
  • Cell Cycle Proteins
  • Stilbenes
  • Resveratrol