Arabidopsis Map Kinase 4 Negatively Regulates Systemic Acquired Resistance

Cell. 2000 Dec 22;103(7):1111-20. doi: 10.1016/s0092-8674(00)00213-0.

Abstract

Transposon inactivation of Arabidopsis MAP kinase 4 produced the mpk4 mutant exhibiting constitutive systemic acquired resistance (SAR) including elevated salicylic acid (SA) levels, increased resistance to virulent pathogens, and constitutive pathogenesis-related gene expression shown by Northern and microarray hybridizations. MPK4 kinase activity is required to repress SAR, as an inactive MPK4 form failed to complement mpk4. Analysis of mpk4 expressing the SA hydroxylase NahG and of mpk4/npr1 double mutants indicated that SAR expression in mpk4 is dependent upon elevated SA levels but is independent of NPR1. PDF1.2 and THI2.1 gene induction by jasmonate was blocked in mpk4 expressing NahG, suggesting that MPK4 is required for jasmonic acid-responsive gene expression.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Alleles
  • Arabidopsis / enzymology*
  • Arabidopsis / growth & development
  • Arabidopsis / microbiology
  • Arabidopsis Proteins*
  • Blotting, Northern
  • Cold Temperature
  • Cyclopentanes / pharmacology
  • Desiccation
  • Gene Expression Regulation, Enzymologic / drug effects
  • Gene Expression Regulation, Enzymologic / physiology
  • Gene Expression Regulation, Plant / drug effects
  • Gene Expression Regulation, Plant / physiology
  • Hot Temperature
  • Immunity, Innate
  • Mutation / physiology
  • Oxylipins
  • Plant Diseases / genetics*
  • Plant Diseases / microbiology
  • Plant Growth Regulators / pharmacology
  • Plant Proteins / genetics*
  • Plant Proteins / metabolism
  • Protein Tyrosine Phosphatases / genetics*
  • Protein Tyrosine Phosphatases / metabolism
  • Pseudomonas
  • Pseudomonas Infections
  • RNA, Messenger / analysis
  • Salts
  • Transcriptional Activation

Substances

  • Arabidopsis Proteins
  • Cyclopentanes
  • NPR1 protein, Arabidopsis
  • Oxylipins
  • Plant Growth Regulators
  • Plant Proteins
  • RNA, Messenger
  • Salts
  • jasmonic acid
  • Protein Tyrosine Phosphatases