The 70-kDa major DNA-compacting protein of the chloroplast nucleoid is sulfite reductase

FEBS Lett. 2001 Jan 5;487(3):347-50. doi: 10.1016/s0014-5793(00)02342-5.

Abstract

The chloroplast nucleoid is a complex of chloroplast DNA and various, mostly uncharacterized proteins. An abundant 70-kDa protein of the isolated nucleoids of pea chloroplasts was identified as sulfite reductase by N-terminal sequence analysis as well as immunoblot analysis, spectrophotometry and enzyme activity analysis. Recombinant maize sulfite reductase was indeed able to compact chloroplast DNA and to form nucleoid-like particles in vitro. The role of sulfite reductase in the structural organization of the nucleoid is discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Chloroplasts / enzymology*
  • Chloroplasts / genetics
  • DNA, Chloroplast / chemistry
  • DNA, Chloroplast / metabolism
  • DNA, Plant / metabolism
  • Molecular Sequence Data
  • Molecular Weight
  • Oxidoreductases Acting on Sulfur Group Donors / chemistry*
  • Oxidoreductases Acting on Sulfur Group Donors / genetics
  • Oxidoreductases Acting on Sulfur Group Donors / metabolism*
  • Peas / enzymology
  • Peas / genetics
  • Plant Proteins / chemistry
  • Plant Proteins / genetics
  • Plant Proteins / metabolism
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Sequence Homology, Amino Acid

Substances

  • DNA, Chloroplast
  • DNA, Plant
  • Plant Proteins
  • Recombinant Proteins
  • Oxidoreductases Acting on Sulfur Group Donors