A quantification of human cells using an ERV-3 real time PCR assay

J Virol Methods. 2001 Feb;91(2):109-17. doi: 10.1016/s0166-0934(00)00244-5.


A novel approach to quantifying human cells using a real time PCR assay was developed. The target sequence used in the assay is a 135 bp segment within the unique 1.7 kb Hind III / Pst I fragment of the ERV-3 envelope gene. ERV-3 is a full-length human endogenous retrovirus present in known copy number in all human cells. The detection range of ERV-3 by real time PCR is from 10(6) to 10(1). The precision described, sensitivity and specificity of the assay indicate that the ERV-3 sequence is an accurate cell quantitation marker. The quantitative ERV-3 assay enables simple, fast, and reproducible detection and quantitation of the cell number. The assay can be used to determine the sample DNA conditions and also it can be used to adjust the quantitative DNA measurements of other target gene assays relative to the number of cell equivalents.

Publication types

  • Evaluation Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Biomarkers
  • Cell Count / methods*
  • Cloning, Molecular
  • DNA Primers / genetics
  • DNA, Viral / blood
  • Endogenous Retroviruses / genetics*
  • Endogenous Retroviruses / isolation & purification
  • Genetic Vectors
  • Humans
  • Mice
  • Polymerase Chain Reaction / methods*
  • Polymerase Chain Reaction / standards
  • Polymerase Chain Reaction / statistics & numerical data
  • Reference Standards
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Templates, Genetic
  • Tumor Cells, Cultured
  • Viral Envelope Proteins / analysis
  • Viral Envelope Proteins / genetics


  • Biomarkers
  • DNA Primers
  • DNA, Viral
  • Viral Envelope Proteins