Syk protein-tyrosine kinase is involved in neuron-like differentiation of embryonal carcinoma P19 cells

FEBS Lett. 2001 Feb 2;489(2-3):129-33. doi: 10.1016/s0014-5793(01)02097-x.


Syk has been implicated in activated immunoreceptors to downstream signaling events in hematopoietic cells. Here we report that Syk is expressed in neuron-like cells and involved in neuron-like differentiation of embryonal carcinoma P19 cells. Immunoblot, RT-PCR, and Northern analysis indicated that Syk is expressed in mouse brain, PC12 and P19 cells. In addition, Syk was found to be tyrosine phosphorylated during neuron-like differentiation of P19 cells. Furthermore, adenovirus-mediated overexpression of Syk induced supernumerary neurite formation and extracellular signal-regulated kinase (ERK) activation in P19 cells. These results suggest that Syk plays an important role in signaling steps leading to ERK activation in P19 cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenoviridae / genetics
  • Animals
  • Brain / cytology
  • Brain / enzymology
  • Carcinoma, Embryonal / enzymology*
  • Carcinoma, Embryonal / pathology
  • Cell Differentiation*
  • DNA, Recombinant
  • Enzyme Activation
  • Enzyme Precursors / genetics
  • Enzyme Precursors / metabolism*
  • Gene Expression Regulation, Enzymologic
  • Intracellular Signaling Peptides and Proteins
  • Mice
  • Mitogen-Activated Protein Kinases / metabolism
  • Neurites / metabolism
  • Neurons / cytology*
  • Neurons / enzymology
  • Phosphorylation
  • Protein-Tyrosine Kinases / genetics
  • Protein-Tyrosine Kinases / metabolism*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Syk Kinase
  • Tumor Cells, Cultured
  • Tyrosine / metabolism


  • DNA, Recombinant
  • Enzyme Precursors
  • Intracellular Signaling Peptides and Proteins
  • RNA, Messenger
  • Tyrosine
  • Protein-Tyrosine Kinases
  • Syk Kinase
  • Syk protein, mouse
  • Mitogen-Activated Protein Kinases