The inhibitory effects of mangiferin, a naturally occurring glucosylxanthone, in bowel carcinogenesis of male F344 rats

Cancer Lett. 2001 Feb 26;163(2):163-70. doi: 10.1016/s0304-3835(00)00678-9.


Mangiferin, 1,3,6,7-tetrahydroxyxanthone-C2-beta-D-glucoside, is one of xanthone derivatives and C-glucosylxanthones, is widely distributed in higher plants and is one of constituents of folk medicines. Recent studies showed that mangiferin has a potential as an anti-oxidant and an anti-viral agent. In this study, we examined the effects of mangiferin in rat colon carcinogenesis induced by chemical carcinogen, azoxymethane (AOM). We performed two experiments: a short-term assay to investigate the effects of mangiferin on the development of preneoplastic lesions by AOM, aberrant crypt foci (ACF), and the following long-term assay for the influence of mangiferin on tumorigenesis induced by AOM. In the short-term assay, 0.1% mangiferin in a diet significantly inhibited the ACF development in rats treated with AOM compared to rats treated with AOM alone (64.6+/-22.0 vs. 108.3+/-43.0). In the long-term assay, the group treated with 0.1% mangiferin in initiation phase of the experimental protocol had significantly lower incidence and multiplicity of intestinal neoplasms induced by AOM (47.3 and 41.8% reductions of the group treated with AOM alone for incidence and multiplicity, respectively). In addition, the cell proliferation in colonic mucosa was reduced in rats treated with mangiferin (65-85% reductions of the group treated with AOM alone). These results suggest that mangiferin has potential as a naturally-occurring chemopreventive agent.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anticarcinogenic Agents / therapeutic use*
  • Azoxymethane
  • Carcinogens
  • Cell Division / drug effects
  • Colonic Neoplasms / chemically induced
  • Colonic Neoplasms / prevention & control*
  • Drug Screening Assays, Antitumor
  • Intestinal Mucosa / drug effects
  • Intestinal Mucosa / pathology
  • Male
  • Precancerous Conditions / chemically induced
  • Precancerous Conditions / prevention & control*
  • Rats
  • Rats, Inbred F344
  • Xanthenes / therapeutic use*
  • Xanthones*


  • Anticarcinogenic Agents
  • Carcinogens
  • Xanthenes
  • Xanthones
  • mangiferin
  • Azoxymethane