An easy and fast non-destructive method for sampling from monument and art object surfaces is proposed. The results obtained after sampling in regions of black spots and discoloration using adhesive tape strips showed that the method is useful for monitoring microbial colonization as well as for the identification of biodeteriogens. This technique is easy to apply, inexpensive and reproducible. The presence of fungi or algae on stone surfaces can be checked at an early stage of colonization or after cleaning procedures, without damage to the sampled surface. In addition, it is possible to obtain information on the morphology and taxonomy of microorganisms, and their relationships with the colonized material surfaces.