2D or not 2D. Two-dimensional gel electrophoresis

Curr Opin Chem Biol. 2001 Feb;5(1):26-33. doi: 10.1016/s1367-5931(00)00167-8.


2D gel electrophoresis is the technology that everyone loves to hate-it requires manual dexterity and precision to reproduce precisely and is thus not well-suited as a high-throughput technology. Although almost everyone would like to replace it, the resolution and sensitivity it offers are exquisite and unsurpassed if one wants a global view of cellular activity. There have been several recent developments, for example, the detection of low abundance proteins, and the resolution possible with narrow-range IPG gels.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Computational Biology
  • Electrophoresis, Gel, Two-Dimensional / methods*
  • Electrophoresis, Gel, Two-Dimensional / standards*
  • Electrophoresis, Gel, Two-Dimensional / trends
  • Humans
  • Proteome / analysis
  • Sensitivity and Specificity


  • Proteome