Large-scale mutagenesis: yeast genetics in the genome era

Curr Opin Biotechnol. 2001 Feb;12(1):28-34. doi: 10.1016/s0958-1669(00)00171-3.


The completion of the DNA sequence of the budding yeast Saccharomyces cerevisiae resulted in the identification of a large number of genes. However, the function of most of these genes is not known. One of the best ways to determine gene function is to carry out mutational and phenotypic analysis. In recent years, several approaches have been developed for the mutational analysis of yeast genes on a large scale. These include transposon-based insertional mutagenesis, and systematic deletions using PCR-based approaches. These projects have produced collections of yeast strains and plasmid alleles that can be screened using novel approaches. Analysis of these collections by the scientific community promises to reveal a great deal of biological information about this organism.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • DNA Mutational Analysis
  • Genes, Essential
  • Genes, Fungal*
  • Molecular Biology / methods*
  • Mutagenesis*
  • Mutagenesis, Insertional
  • Saccharomyces cerevisiae / genetics*
  • Sequence Deletion