Identification of a novel DRB1 allele, DRB1*1112, by sequence-based DRB typing

Tissue Antigens. 2001 Jan;57(1):85-6. doi: 10.1034/j.1399-0039.2001.057001085.x.

Abstract

We report here a novel DRB1 allele (DRB1*1112) identified during sequence-based HLA-DRB typing. Polymerase chain reaction with generic DRB primers and group-specific primers and subsequent sequencing yielded identical results. Molecular cloning and sequencing confirmed that the new DRB1 allele is identical to DRB1*11011 and 1129 at exon 2 except for a single nucleotide substitution at codon 37, changing the codon from Tyr (DRB1*11011) or Ser (DRB1*1129) to Phe.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Alleles*
  • Base Sequence
  • HLA-DR Antigens / genetics*
  • HLA-DRB1 Chains
  • Histocompatibility Testing / methods*
  • Humans
  • Male
  • Molecular Sequence Data
  • Sequence Analysis, DNA
  • White People / genetics

Substances

  • HLA-DR Antigens
  • HLA-DRB1 Chains
  • HLA-DRB1*11:01:01 antigen
  • HLA-DRB1*12:12 antigen

Associated data

  • GENBANK/AF234175