Analysis of the molecular mechanism involved in 2B4-mediated NK cell activation: evidence that human 2B4 is physically and functionally associated with the linker for activation of T cells

Eur J Immunol. 2000 Dec;30(12):3718-22. doi: 10.1002/1521-4141(200012)30:12<3718::AID-IMMU3718>3.0.CO;2-I.

Abstract

While 2B4 is a well-known surface receptor involved in NK cell triggering and induction of cytotoxicity against CD48-positive target cells, little is known about the downstream events which lead to NK cell activation. In this study we show that, in normal human NK cells, 2B4 constitutively associates with the linker for activation of T cells (LAT). Antibody-mediated engagement of 2B4 resulted in tyrosine phosphorylation not only of 2B4 but also of the associated LAT molecules. Moreover, tyrosine phosphorylation of LAT led to the recruitment of intracytoplasmic signaling molecules including phospholipase Cgamma and Grb2. These data support the concept that 2B4 may mediate NK cell triggering via a LAT-dependent signaling pathway.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, CD*
  • Humans
  • Killer Cells, Natural / immunology*
  • Lymphocyte Activation*
  • Membrane Glycoproteins / physiology*
  • Phosphorylation
  • Receptors, Immunologic*
  • Signaling Lymphocytic Activation Molecule Family
  • Type C Phospholipases / physiology
  • Tyrosine / metabolism

Substances

  • Antigens, CD
  • CD244 protein, human
  • Membrane Glycoproteins
  • Receptors, Immunologic
  • Signaling Lymphocytic Activation Molecule Family
  • Tyrosine
  • Type C Phospholipases