Enhanced neurogenesis in the rodent hippocampus following traumatic brain injury

J Neurosci Res. 2001 Feb 15;63(4):313-9. doi: 10.1002/1097-4547(20010215)63:4<313::AID-JNR1025>3.0.CO;2-4.


Recent studies have shown that neurogenesis in the dentate gyrus of the rodent hippocampus continues throughout life. Several physiological and pathological conditions have been reported to alter the rate of progenitor cell division resulting in the increased production of mature granule neurons. Excitotoxic and mechanical lesions of the granule cell layer also stimulate the proliferation of precursor cells suggesting that the death of pre-existing granule neurons may act as a trigger for enhanced neurogenesis. Hippocampal pyramidal neurons, and to a lesser extent granule neurons, have been reported to die as a result of traumatic brain injury in rodents. To determine if the proliferation of precursor cells is enhanced as a result of brain injury in rodents, newly divided cells were labeled with the thymidine analog, bromodeoxyuridine (BrdU). Traumatic brain injury increased the production of BrdU-labeled cells in the dentate gyrus with a maximal rate observed at 3 days post-injury. These cells, a proportion of which co-localize with the immature neuronal marker TOAD-64, implanted themselves into the granule cell layer where they accumulated over time. When examined 1 month post-injury, the majority of BrdU-labeled cells co-labeled with the mature neuronal marker calbindin. These findings show that traumatic brain injury increases neurogenesis in the granule cell layer and suggests that these new cells may contribute to the function of the hippocampus.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Biomarkers
  • Brain Injuries / pathology*
  • Brain Injuries / physiopathology
  • Bromodeoxyuridine / analysis
  • Cell Differentiation / physiology
  • Cerebral Cortex / injuries*
  • Cerebral Cortex / pathology
  • Dentate Gyrus / cytology*
  • In Situ Nick-End Labeling
  • Male
  • Nerve Tissue Proteins / analysis
  • Neurons / chemistry
  • Neurons / cytology*
  • Rats
  • Rats, Long-Evans
  • Stem Cells / cytology


  • Biomarkers
  • Nerve Tissue Proteins
  • Bromodeoxyuridine