A cluster of three novel Ca2+ channel gamma subunit genes on chromosome 19q13.4: evolution and expression profile of the gamma subunit gene family

Genomics. 2001 Feb 1;71(3):339-50. doi: 10.1006/geno.2000.6440.


The CACNG1 gene on chromosome 17q24 encodes an integral membrane protein that was originally isolated as the regulatory gamma subunit of voltage-dependent Ca2+ channels from skeletal muscle. The existence of an extended family of gamma subunits was subsequently demonstrated upon identification of CACNG2 (22q13), CACNG3 (16p12-p13), and CACNG4 and CACNG5 (17q24). In this study, we describe a cluster of three novel gamma subunit genes, CACNG6, CACNG7, and CACNG8, located in a tandem array on 19q13.4. Phylogenetic analysis indicates that this array is paralogous to the cluster containing CACNG1, CACNG5, and CACNG4, respectively, on chromosome 17q24. We developed sensitive RT-PCR assays and examined the expression profile of each member of the gamma subunit gene family, CACNG1-CACNG8. Analysis of 24 human tissues plus 3 dissected brain regions revealed that CACNG1 through CACNG8 are all coexpressed in fetal and adult brain and differentially transcribed among a wide variety of other tissues. The expression of distinct complements of gamma subunit isoforms in different cell types may be an important mechanism for regulating Ca2+ channel function.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Brain / embryology
  • Brain / metabolism
  • Calcium Channels / genetics*
  • Cell Line
  • Cerebellum / metabolism
  • Cerebral Cortex / metabolism
  • Chromosomes, Human, Pair 19*
  • Cloning, Molecular
  • Evolution, Molecular
  • Gene Expression Regulation
  • Genome
  • Hippocampus / metabolism
  • Humans
  • Models, Genetic
  • Molecular Sequence Data
  • Multigene Family*
  • Muscle, Skeletal / metabolism
  • Phylogeny
  • Protein Isoforms
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Homology, Amino Acid
  • Tissue Distribution
  • Transcription, Genetic


  • Calcium Channels
  • Protein Isoforms

Associated data

  • GENBANK/AF288386
  • GENBANK/AF288387
  • GENBANK/AF288388