Prolonged measles virus shedding in human immunodeficiency virus-infected children, detected by reverse transcriptase-polymerase chain reaction

J Infect Dis. 2001 Feb 15;183(4):532-8. doi: 10.1086/318533. Epub 2001 Jan 18.

Abstract

A reverse transcriptase-polymerase chain reaction assay was used to detect measles virus RNA in peripheral blood mononuclear cells, urine, and nasopharyngeal specimens from Zambian children during hospitalization and approximately 1-2 months after discharge. Of 47 children, 29 (61.7%) had prolonged measles virus shedding, as defined by detection of measles virus RNA in > or =1 specimen obtained 30-61 days after rash onset. Ten (90.9%) of 11 human immunodeficiency virus (HIV)-infected children had prolonged measles virus shedding, compared with 19 (52.8%) of 36 HIV-uninfected children (P=.02). Prolonged measles virus shedding did not correlate with levels of measles virus-specific antibody. HIV-infected children with measles may have a prolonged infectious period that potentially enhances measles virus transmission and hinders measles control.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adolescent
  • Antibodies, Viral / blood
  • Child
  • Child, Preschool
  • Female
  • HIV Infections / complications*
  • HIV Seronegativity
  • HIV-1 / isolation & purification
  • Humans
  • Infant
  • Leukocytes, Mononuclear / virology
  • Male
  • Measles / complications*
  • Measles / virology
  • Measles virus / genetics
  • Measles virus / immunology
  • Measles virus / isolation & purification*
  • Measles virus / physiology
  • Nasopharynx / virology
  • RNA, Viral / analysis
  • Reverse Transcriptase Polymerase Chain Reaction
  • Time Factors
  • Urine / virology
  • Virus Shedding* / physiology
  • Zambia

Substances

  • Antibodies, Viral
  • RNA, Viral