TUP1 utilizes histone H3/H2B-specific HDA1 deacetylase to repress gene activity in yeast

Mol Cell. 2001 Jan;7(1):117-26. doi: 10.1016/s1097-2765(01)00160-5.

Abstract

TUP1 is recruited to and represses genes that regulate mating, glucose and oxygen use, stress response, and DNA damage. It is shown here that disruption of either TUP1 or histone deacetylase HDA1 causes histone H3/H2B--specific hyperacetylation next to the TUP1 binding site at the stress-responsive ENA1 promoter. It is also shown that TUP1 interacts with HDA1 in vitro. These data indicate that TUP1 mediates localized histone deacetylation through HDA1. Interestingly, RPD3 deacetylates the ENA1 coding region, and both deacetylases contribute to ENA1 repression. However, epistasis analysis argues that only HDA1 and TUP1 are likely to function in the same pathway. These data define gene and histone targets of HDA1 and illustrate the role of histone deacetylation in TUP1 repression.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • ATP-Binding Cassette Transporters / genetics
  • ATP-Binding Cassette Transporters / metabolism
  • Acetylation
  • Adenosine Triphosphatases / genetics
  • Adenosine Triphosphatases / metabolism
  • Antigens, Bacterial
  • Antigens, Fungal
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Cation Transport Proteins*
  • Cytochrome c Group / genetics
  • Cytochrome c Group / metabolism
  • Cytochromes c*
  • Drug Combinations
  • Fungal Proteins / genetics*
  • Fungal Proteins / metabolism*
  • Gene Expression Regulation, Fungal
  • Glycoproteins*
  • Histone Deacetylases / genetics
  • Histone Deacetylases / metabolism*
  • Histones / metabolism*
  • Lac Operon
  • Mutagenesis / physiology
  • Nuclear Proteins*
  • Promoter Regions, Genetic / physiology
  • Repressor Proteins*
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins*
  • Serine Endopeptidases / genetics
  • Serine Endopeptidases / metabolism
  • Sodium-Potassium-Exchanging ATPase
  • Suppression, Genetic / physiology*
  • Thimerosal
  • Transcription Factors / genetics
  • Transcription Factors / metabolism

Substances

  • ATP-Binding Cassette Transporters
  • Antigens, Bacterial
  • Antigens, Fungal
  • Bacterial Proteins
  • CYC1 protein, S cerevisiae
  • Cation Transport Proteins
  • Cytochrome c Group
  • Drug Combinations
  • ENA1 protein, S cerevisiae
  • Fungal Proteins
  • Glycoproteins
  • Histones
  • LexA protein, Bacteria
  • Nuclear Proteins
  • Repressor Proteins
  • STE6 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • TUP1 protein, S cerevisiae
  • Transcription Factors
  • imudon
  • Thimerosal
  • Cytochromes c
  • Serine Endopeptidases
  • RPD3 protein, S cerevisiae
  • Histone Deacetylases
  • Adenosine Triphosphatases
  • Sodium-Potassium-Exchanging ATPase