Specific alterations of U1-C protein or U1 small nuclear RNA can eliminate the requirement of Prp28p, an essential DEAD box splicing factor

Mol Cell. 2001 Jan;7(1):227-32. doi: 10.1016/s1097-2765(01)00170-8.


While some members of the ubiquitous DExD/H box family of proteins have RNA helicase activity in vitro, their roles in vivo remain virtually unknown. Here, we show that the function of an otherwise essential DEAD box protein, Prp28p, can be bypassed by mutations that alter either the protein U1-C or the U1 small nuclear RNA. Further analysis suggests that the conserved L13 residue in the U1-C protein makes specific contact to stabilize the U1 snRNA/5' splice site duplex in the prespliceosome, and that Prp28p functions to counteract the stabilizing effect of the U1-C protein, thereby promoting the dissociation of the U1 small nuclear ribonucleoprotein particle from the 5' splice site. Thus, in addition to unwinding RNA, the DExD/H box proteins may affect RNA-RNA rearrangements by antagonizing specific RNA-stabilizing proteins.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • DEAD-box RNA Helicases
  • Molecular Sequence Data
  • Mutation / genetics
  • RNA Nucleotidyltransferases / genetics*
  • RNA Nucleotidyltransferases / metabolism
  • RNA Splicing / physiology*
  • Ribonucleoprotein, U1 Small Nuclear / genetics*
  • Ribonucleoprotein, U1 Small Nuclear / metabolism
  • Ribonucleoproteins, Small Nuclear / genetics*
  • Ribonucleoproteins, Small Nuclear / metabolism
  • Saccharomyces cerevisiae Proteins
  • Sequence Homology, Amino Acid
  • Suppression, Genetic / physiology
  • Yeasts


  • Ribonucleoprotein, U1 Small Nuclear
  • Ribonucleoproteins, Small Nuclear
  • Saccharomyces cerevisiae Proteins
  • RNA Nucleotidyltransferases
  • PRP28 protein, S cerevisiae
  • DEAD-box RNA Helicases