Opposing regulation of B cell receptor-induced activation of mitogen-activated protein kinases by CD45

FEBS Lett. 2001 Feb 9;490(1-2):97-101. doi: 10.1016/s0014-5793(00)02416-9.


In this study, we examined the contribution made by CD45 to B cell antigen receptor (BCR)-induced activation of mitogen-activated protein kinase (MAPK) family members. We found that CD45 negatively regulated BCR-induced c-Jun NH(2)-terminal kinase (JNK) and p38 activation in immature WEHI-231 cells, whereas in mature BAL-17 cells, CD45 positively regulated JNK and p38 activation and negatively regulated extracellular signal-regulated kinase activity. Furthermore, cooperative action of JNK and p38 dictated BCR-induced inhibition of growth. Thus, CD45 appears to differentially regulate BCR-induced activation of MAPK members, and can exert opposing effects on JNK and p38 in different cellular milieu, controlling the B cell fate.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Western
  • Cell Line
  • Cell Lineage
  • Enzyme Activation
  • Enzyme Inhibitors / pharmacology
  • Flavonoids / pharmacology
  • Imidazoles / pharmacology
  • JNK Mitogen-Activated Protein Kinases
  • Leukocyte Common Antigens / physiology*
  • MAP Kinase Signaling System*
  • Mice
  • Mitogen-Activated Protein Kinases / metabolism
  • Pyridines / pharmacology
  • Receptors, Antigen, B-Cell / metabolism*
  • Time Factors
  • Transfection
  • p38 Mitogen-Activated Protein Kinases


  • Enzyme Inhibitors
  • Flavonoids
  • Imidazoles
  • Pyridines
  • Receptors, Antigen, B-Cell
  • JNK Mitogen-Activated Protein Kinases
  • Mitogen-Activated Protein Kinases
  • p38 Mitogen-Activated Protein Kinases
  • Leukocyte Common Antigens
  • SB 203580
  • 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one