Successful pregnancy and delivery from frozen-thawed embryos after intracytoplasmic sperm injection using round-headed spermatozoa and assisted oocyte activation in a globozoospermic patient with mosaic Down syndrome

Fertil Steril. 2001 Feb;75(2):445-7. doi: 10.1016/s0015-0282(00)01698-8.

Abstract

Objective: To describe a successful pregnancy and delivery from frozen-thawed embryos after intracytoplasmic sperm injection (ICSI) and assisted oocyte activation in a globozoospermic patient with mosaic Down syndrome.

Design: Controlled clinical study.

Setting: IVF Laboratory, PL Infertility Clinic, Seoul, Korea.

Patient(s): A couple with infertility resulting from globozoospermia with mosaic Down syndrome: 47,XY,+21[7]/46,XY[33].

Intervention(s): Semen analysis, karyotyping, ICSI, assisted oocyte activation, assisted hatching, and frozen-thawed ET.

Main outcome measure(s): Fertilization rate, implantation, pregnancy, and delivery.

Result(s): Thirty-eight oocytes were aspirated, and round-headed spermatozoa were injected into 35 oocytes in metaphase II. Assisted oocyte activation with calcium ionophore A23187 after ICSI resulted in a high fertilization rate (21 of 35, 60%; 2 pronuclei in 18 of 21; 3 pronuclei in 3 of 21) and good embryo development. At 3 days after ICSI, 5 embryos of good quality were surgically transferred to the endometrium after assisted hatching, but no pregnancy occurred. After 2 months, the surgical transfer of 4 frozen-thawed embryos after assisted hatching led to an ongoing pregnancy. A female infant weighing 3,000 g was delivered at 38 weeks of gestation by cesarean section.

Conclusion(s): We report the first successful pregnancy and delivery from frozen-thawed embryos after ICSI and assisted oocyte activation in a globozoospermic patient with mosaic Down syndrome.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Calcimycin / pharmacology
  • Cesarean Section
  • Cryopreservation*
  • Down Syndrome / complications*
  • Down Syndrome / genetics
  • Embryo Implantation
  • Embryo Transfer
  • Embryo, Mammalian / physiology
  • Female
  • Humans
  • Infertility, Male / complications
  • Infertility, Male / therapy*
  • Ionophores / pharmacology
  • Male
  • Mosaicism
  • Oocytes / drug effects
  • Oocytes / physiology
  • Pregnancy
  • Pregnancy Outcome*
  • Sperm Head
  • Sperm Injections, Intracytoplasmic*
  • Spermatozoa / abnormalities*

Substances

  • Ionophores
  • Calcimycin