Interphase movements of a DNA chromosome region modulated by VP16 transcriptional activator

Nat Cell Biol. 2001 Feb;3(2):134-9. doi: 10.1038/35055033.


We examined changes in intranuclear chromosome positioning induced by a transcriptional activator in a simple experimental system. Targeting the VP16 acidic activation domain (AAD) to an engineered chromosome site resulted in its transcriptional activation and redistribution from a predominantly peripheral to a more interior nuclear localization. Direct visualization in vivo revealed that the chromosome site normally moves into the nuclear interior transiently in early G1 and again in early S phase. In contrast, VP16 AAD targeting induced this site's permanent interior localization in early G1. A single transcriptional activator therefore can modify the cell-cycle-dependent programme of intranuclear positioning of chromosome loci.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Blotting, Northern
  • Cell Line
  • Cell Nucleus / genetics
  • Cell Nucleus / metabolism*
  • Chromosomes / genetics*
  • Chromosomes / metabolism
  • Cricetinae
  • DNA / genetics
  • DNA / metabolism*
  • Genes, Reporter / genetics
  • Herpes Simplex Virus Protein Vmw65 / genetics*
  • Herpes Simplex Virus Protein Vmw65 / metabolism
  • Interphase*
  • Microscopy, Fluorescence
  • Protein Structure, Tertiary*
  • Rats
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Regulatory Sequences, Nucleic Acid / genetics
  • Transcriptional Activation / genetics


  • Herpes Simplex Virus Protein Vmw65
  • Recombinant Fusion Proteins
  • DNA