Cloning and characterization of a novel ITIM containing lectin-like immunoreceptor LLIR and its two transmembrane region deletion variants

Biochem Biophys Res Commun. 2001 Feb 16;281(1):131-40. doi: 10.1006/bbrc.2001.4322.


A novel full-length cDNA was cloned from human dendritic cells (DC) by subtractive cloning and RACE. The deduced protein is a type II lectin-like membrane protein that contains an ITIM proximal to N terminal and is designated as lectin-like immunoreceptor (LLIR). The gene of LLIR is located in a region of chromosomal 12p13 and shows highest homologous with ASGPR. Two alternatively spliced transmembraneless variants of LLIR were identified by RT-PCR and named as LLIRv1 and LLIRv2. RT-PCR and immunoblotting analysis revealed that LLIR was expressed with much higher level in immature DC than in mature DC. The ITIM in LLIR was demonstrated to bind SHP-1 in HL-60 cell after the tyrosine had been phosphorylated. In addition, the mRNA expression level of LLIRv2 was raised when leukemia cells were induced to differentiate by PMA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3 Cells
  • Alternative Splicing
  • Amino Acid Sequence
  • Animals
  • B-Lymphocytes / metabolism
  • Base Sequence
  • Blotting, Northern
  • Carcinogens
  • Cell Differentiation
  • Cell Line
  • Cell Membrane / metabolism
  • Chromosome Mapping
  • Chromosomes, Human, Pair 12
  • Cloning, Molecular
  • DNA, Complementary / metabolism
  • Dendritic Cells / metabolism
  • Enzyme Inhibitors / pharmacology
  • Gene Deletion
  • HL-60 Cells
  • Humans
  • Immunoblotting
  • Lectins / chemistry*
  • Lectins, C-Type*
  • Membrane Glycoproteins*
  • Mice
  • Molecular Sequence Data
  • Phosphorylation
  • Protein Binding
  • Protein Biosynthesis
  • RNA, Messenger / metabolism
  • Receptors, Immunologic / biosynthesis
  • Receptors, Immunologic / chemistry*
  • Receptors, Immunologic / genetics*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Homology, Amino Acid
  • Tetradecanoylphorbol Acetate
  • Tissue Distribution
  • Tumor Cells, Cultured
  • Up-Regulation
  • Vanadates / pharmacology


  • CLEC4A protein, human
  • Carcinogens
  • DNA, Complementary
  • Enzyme Inhibitors
  • Lectins
  • Lectins, C-Type
  • Membrane Glycoproteins
  • RNA, Messenger
  • Receptors, Immunologic
  • pervanadate
  • Vanadates
  • Tetradecanoylphorbol Acetate