Remodeling of yeast genome expression in response to environmental changes

Mol Biol Cell. 2001 Feb;12(2):323-37. doi: 10.1091/mbc.12.2.323.


We used genome-wide expression analysis to explore how gene expression in Saccharomyces cerevisiae is remodeled in response to various changes in extracellular environment, including changes in temperature, oxidation, nutrients, pH, and osmolarity. The results demonstrate that more than half of the genome is involved in various responses to environmental change and identify the global set of genes induced and repressed by each condition. These data implicate a substantial number of previously uncharacterized genes in these responses and reveal a signature common to environmental responses that involves approximately 10% of yeast genes. The results of expression analysis with MSN2/MSN4 mutants support the model that the Msn2/Msn4 activators induce the common response to environmental change. These results provide a global description of the transcriptional response to environmental change and extend our understanding of the role of activators in effecting this response.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adaptation, Physiological / genetics*
  • DNA-Binding Proteins / genetics
  • Enzymes / genetics
  • Gene Expression Profiling
  • Gene Expression Regulation, Enzymologic
  • Gene Expression Regulation, Fungal* / drug effects
  • Genome, Fungal
  • Heat-Shock Response / genetics
  • Hydrogen Peroxide / pharmacology
  • Hydrogen-Ion Concentration
  • Mutation
  • Osmotic Pressure
  • Saccharomyces cerevisiae Proteins*
  • Salts / pharmacology
  • Transcription Factors / genetics
  • Yeasts / drug effects
  • Yeasts / physiology*


  • DNA-Binding Proteins
  • Enzymes
  • MSN2 protein, S cerevisiae
  • MSN4 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Salts
  • Transcription Factors
  • Hydrogen Peroxide