Discovery and analysis of antisense oligonucleotide activity in cell culture

Methods. 2001 Feb;23(2):191-8. doi: 10.1006/meth.2000.1120.


In the past decade antisense oligonucleotides (ASOs) have proven to be a useful tool for dissection of gene function in molecular cell biology (Koller, E., Gaarde, W. A., and Monia, B. P. (2000) Trends Pharm. Sci., 21, 142-148), and validation of gene targets in animal models (Crooke, S. T. (1998) Biotechnol. Gen. Eng. Rev. 15, 121-157), as well as a means for therapeutic treatment of human diseases (Bennett, C. F. (1999) Exp. Opin. Invest. Drugs 8, 237-253). An important step toward usage of ASOs in the described applications is identification of an active ASO. This article describes the underlying basis and means for achieving this goal in cell culture.

Publication types

  • Review

MeSH terms

  • Animals
  • Blotting, Northern
  • Cell Line
  • Cells, Cultured
  • Genetic Techniques*
  • Humans
  • Oligonucleotide Array Sequence Analysis
  • Oligonucleotides, Antisense / metabolism*
  • RNA / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Ribonuclease H / metabolism
  • Ribonucleases / metabolism


  • Oligonucleotides, Antisense
  • RNA
  • Ribonucleases
  • Ribonuclease H