A high-resolution radiation hybrid map of the human genome draft sequence

Science. 2001 Feb 16;291(5507):1298-302. doi: 10.1126/science.1057437.


We have constructed a physical map of the human genome by using a panel of 90 whole-genome radiation hybrids (the TNG panel) in conjunction with 40,322 sequence-tagged sites (STSs) derived from random genomic sequences as well as expressed sequences. Of 36,678 STSs on the TNG radiation hybrid map, only 3604 (9.8%) were absent from the unassembled draft sequence of the human genome. Of 20,030 STSs ordered on the TNG map as well as the assembled human genome draft sequence and the Celera assembled human genome sequence, 36% of the STSs had a discrepant order between the working draft sequence and the Celera sequence. The TNG map order was identical to one of the two sequence orders in 60% of these discrepant cases.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Algorithms
  • Chromosomes, Artificial, Bacterial
  • Computational Biology
  • Contig Mapping
  • Databases, Factual
  • Genome, Human*
  • Human Genome Project
  • Humans
  • In Situ Hybridization, Fluorescence
  • Physical Chromosome Mapping
  • Polymerase Chain Reaction
  • Radiation Hybrid Mapping*
  • Sequence Analysis, DNA*
  • Sequence Tagged Sites
  • Software