Locked nucleic acid (LNA): fine-tuning the recognition of DNA and RNA

Chem Biol. 2001 Jan;8(1):1-7. doi: 10.1016/s1074-5521(00)00058-2.

Abstract

Locked nucleic acid is an RNA derivative in which the ribose ring is constrained by a methylene linkage between the 2'-oxygen and the 4'-carbon. This conformation restriction increases binding affinity for complementarity sequences and provides an exciting new chemical approach for the control of gene expression and optimization of microarrays.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Animals
  • Binding Sites
  • DNA / chemistry*
  • DNA / genetics
  • DNA / metabolism
  • Drug Stability
  • Enzyme Activation
  • Gene Expression
  • In Vitro Techniques
  • Nucleic Acids / chemistry*
  • Nucleic Acids / metabolism
  • Oligonucleotide Array Sequence Analysis
  • Peptide Nucleic Acids / chemistry
  • Peptide Nucleic Acids / metabolism
  • RNA / chemistry*
  • RNA / genetics
  • RNA / metabolism
  • Ribonuclease H / metabolism

Substances

  • Nucleic Acids
  • Peptide Nucleic Acids
  • RNA
  • DNA
  • Ribonuclease H