RNA synthesis in starved deciliated Tetrahymena pyriformis

Eur J Biochem. 1979 Jul;98(1):267-73. doi: 10.1111/j.1432-1033.1979.tb13185.x.

Abstract

Tetrahymena pyriformis which has been starved for 20 h by incubation in buffer, and then deciliated, can regenerate its cilia in about 90 min while still in suspension in non-nutrient medium. The process of reciliation is accompanied by protein synthesis which begins a few minutes after deciliation and by synthesis of ribosomal and messenger RNAs during a period extending from about 1 h to about 3 h after deciliation. Although net synthesis of RNA remains at a very low level until 1 h after deciliation, a qualitative change in the translatable poly(A)-containing messenger RNA content of deciliated cells, and in particular, formation of beta-tubulin mRNA can be detected almost immediately after deciliation.

MeSH terms

  • Animals
  • Cilia / physiology*
  • Polyribosomes / metabolism
  • RNA / biosynthesis*
  • Tetrahymena pyriformis / metabolism*
  • Tetrahymena pyriformis / physiology
  • Transcription, Genetic*

Substances

  • RNA