Abstract
In the study reported in this paper, sensitive ELISA for rat CgA was developed using synthetic rat CgA(359-389) as antigen, N alpha-biotinylated glycylglycyl rat CgA(359-389), and antirat CgA(359-389) serum for the measurement of CgA-LI in rat saliva. CgA-LI in rat submandibular tissues and saliva was characterized by both immunohistochemical and immunochemical methods. Using isolated perfused rat submandibular gland. VIP at 0.1-1.0 nM in the presence of 0.1 microM ACh was found to cause CgA-LI secretion, whereas neither PACAP-27 nor PACAP-38 showed any effect on CgA secretion.
MeSH terms
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Amino Acid Sequence
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Animals
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Chromogranin A
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Chromogranins / chemistry
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Chromogranins / metabolism*
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Enzyme-Linked Immunosorbent Assay / methods
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Immunohistochemistry
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In Vitro Techniques
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Molecular Sequence Data
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Neuropeptides / metabolism
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Neuropeptides / pharmacology*
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Peptide Fragments / chemistry
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Perfusion
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Pituitary Adenylate Cyclase-Activating Polypeptide
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Rats
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Saliva / metabolism
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Submandibular Gland / drug effects*
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Submandibular Gland / metabolism*
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Vasoactive Intestinal Peptide / metabolism
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Vasoactive Intestinal Peptide / pharmacology*
Substances
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Adcyap1 protein, rat
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Chromogranin A
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Chromogranins
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Neuropeptides
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Peptide Fragments
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Pituitary Adenylate Cyclase-Activating Polypeptide
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chromogranin A, rat
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Vasoactive Intestinal Peptide