VIP- and PACAP-induced salivary chromogranin A secretion in the isolated perfused submandibular gland of rats

Ann N Y Acad Sci. 2000:921:218-25. doi: 10.1111/j.1749-6632.2000.tb06969.x.

Abstract

In the study reported in this paper, sensitive ELISA for rat CgA was developed using synthetic rat CgA(359-389) as antigen, N alpha-biotinylated glycylglycyl rat CgA(359-389), and antirat CgA(359-389) serum for the measurement of CgA-LI in rat saliva. CgA-LI in rat submandibular tissues and saliva was characterized by both immunohistochemical and immunochemical methods. Using isolated perfused rat submandibular gland. VIP at 0.1-1.0 nM in the presence of 0.1 microM ACh was found to cause CgA-LI secretion, whereas neither PACAP-27 nor PACAP-38 showed any effect on CgA secretion.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Chromogranin A
  • Chromogranins / chemistry
  • Chromogranins / metabolism*
  • Enzyme-Linked Immunosorbent Assay / methods
  • Immunohistochemistry
  • In Vitro Techniques
  • Molecular Sequence Data
  • Neuropeptides / metabolism
  • Neuropeptides / pharmacology*
  • Peptide Fragments / chemistry
  • Perfusion
  • Pituitary Adenylate Cyclase-Activating Polypeptide
  • Rats
  • Saliva / metabolism
  • Submandibular Gland / drug effects*
  • Submandibular Gland / metabolism*
  • Vasoactive Intestinal Peptide / metabolism
  • Vasoactive Intestinal Peptide / pharmacology*

Substances

  • Adcyap1 protein, rat
  • Chromogranin A
  • Chromogranins
  • Neuropeptides
  • Peptide Fragments
  • Pituitary Adenylate Cyclase-Activating Polypeptide
  • chromogranin A, rat
  • Vasoactive Intestinal Peptide