Inducible expression and cellular location of AgrB, a protein involved in the maturation of the staphylococcal quorum-sensing pheromone

Arch Microbiol. 2000 Dec;174(6):452-5. doi: 10.1007/s002030000223.


AgrB has been suggested to be responsible for the posttranslational modification in staphylococci that leads to the production of the thiolactone-containing agr peptide pheromone. We demonstrate that AgrB is located in the cytoplasmic membrane. Vectors were constructed for the xylose-inducible overexpression of agrB, and of agrB and agrD together. A Staphylococcus epidermidis strain deleted for agr and containing these vectors was assayed for AgrB protein and pheromone production. The lack of adequate pheromone production suggests the involvement of additional factors in the production of the agr pheromone.

MeSH terms

  • Bacterial Proteins / genetics*
  • Bacterial Proteins / metabolism*
  • Cell Membrane / metabolism
  • Culture Media
  • Gene Expression Regulation, Bacterial
  • Peptides, Cyclic / metabolism*
  • Pheromones / metabolism*
  • Plasmids
  • Staphylococcus epidermidis / genetics*
  • Staphylococcus epidermidis / metabolism
  • Trans-Activators*
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism*
  • Transformation, Genetic


  • Agr protein, Staphylococcus aureus
  • Bacterial Proteins
  • Culture Media
  • Peptides, Cyclic
  • Pheromones
  • Trans-Activators
  • Transcription Factors