Estimation of microsatellite mutation rates in Drosophila melanogaster

Genet Res. 2000 Dec;76(3):323-6. doi: 10.1017/s0016672300004791.

Abstract

Microsatellite mutations were studied in a set of 175 mutation accumulation lines, all of them independently derived from a completely homozygous population of Drosophila melanogaster and maintained under strong inbreeding during 80 generations. We assayed 28 microsatellites and detected two mutations. One mutation consisted of a single addition of a dinucleotide repeat and the other was a deletion of five trinucleotide repeats. The average mutation rate was 5.1 x 10(-6), in full agreement with previous estimates from two different sets of mutation accumulation lines.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • DNA / analysis
  • Drosophila melanogaster / genetics*
  • Microsatellite Repeats / genetics*
  • Mutation / genetics*
  • Plasmids
  • Polymerase Chain Reaction

Substances

  • DNA