Maternal exposure to nitrate from drinking water and diet and risk for neural tube defects

Am J Epidemiol. 2001 Feb 15;153(4):325-31. doi: 10.1093/aje/153.4.325.


In this population-based case-control study conducted in California between June 1989 and May 1991, the authors investigated the association between maternal periconceptional exposure to nitrate from drinking water and diet and risk for neural tube defects. The mothers of 538 cases and 539 nonmalformed controls were interviewed regarding residential history, consumption of tap water at home, and dietary intake during the periconceptional period. Dietary nitrate exposure was not associated with increased risk for neural tube defects. Exposure to nitrate in drinking water at concentrations above the 45 mg/liter maximum contaminant level was associated with increased risk for anencephaly (odds ratio (OR) = 4.0, 95% confidence interval (CI): 1.0, 15.4), but not for spina bifida. Increased risks for anencephaly were observed at nitrate levels below the maximum contaminant level among groundwater drinkers only (OR = 2.1, 95% CI: 1.1,4.1 for 5-15 mg/liter; OR = 2.3, 95% CI: 1.1, 4.5 for 16-35 mg/liter; and OR = 6.9, 95% CI: 1.9, 24.9 for 36-67 mg/liter compared with <5 mg/liter). Adjustment for identified risk factors for anencephaly did not substantially alter these associations, nor did control for maternal dietary nitrate, total vitamin C intake, and quantity of tap water consumed. The lack of an observed elevation in risk for anencephaly in association with exposure to mixed water containing nitrate at levels comparable with the concentration in groundwater may indicate that something other than nitrate accounts for these findings.

MeSH terms

  • Adolescent
  • Adult
  • Anencephaly / chemically induced
  • Anencephaly / epidemiology
  • California / epidemiology
  • Case-Control Studies
  • Diet / adverse effects*
  • Female
  • Humans
  • Maternal Exposure / adverse effects*
  • Multivariate Analysis
  • Neural Tube Defects / chemically induced*
  • Neural Tube Defects / epidemiology
  • Nitrates / adverse effects*
  • Pregnancy
  • Risk Factors
  • Spinal Dysraphism / chemically induced
  • Spinal Dysraphism / epidemiology
  • Water Pollutants / adverse effects*
  • Water Supply* / analysis


  • Nitrates
  • Water Pollutants