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Comparative Study
, 1 (1), 75-87

Functional Analysis of the Pseudomonas Syringae rulAB Determinant in Tolerance to Ultraviolet B (290-320 Nm) Radiation and Distribution of rulAB Among P. Syringae Pathovars

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Comparative Study

Functional Analysis of the Pseudomonas Syringae rulAB Determinant in Tolerance to Ultraviolet B (290-320 Nm) Radiation and Distribution of rulAB Among P. Syringae Pathovars

G W Sundin et al. Environ Microbiol.

Abstract

The effect of the plasmid-encoded rulAB (resistance to ultraviolet radiation) determinant on responses of Pseudomonas syringae to ultraviolet-B (UV-B) radiation and the distribution of rulAB among pathovars of P. syringae were determined. The cloned rulAB determinant and the native rulAB+ plasmid pPSR1 both conferred approximately a 10-fold increase in survival on P. syringae pv. syringae FF5 following increasing doses of UV-B radiation. rulAB+ P. syringae strains also maintained significantly larger epiphytic populations on leaf surfaces irradiated with UV-B. rulAB-insertional mutants, constructed in two native rulAB+ strains, were from 10- to 100-fold more sensitive to UV-B radiation. The UV tolerance phenotype and the rulAB genes were widely distributed among P. syringae pathovars isolated from varied plant hosts throughout the world and within a broad range of genotypic backgrounds of P. syringae pv. syringae. With one exception, the rulAB determinant was harboured on pPT23A-like plasmids; these replicons are indigenous residents of the species P. syringae and also tend to encode determinants of importance in host-pathogen interactions.

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