Watching proteins in the wild: fluorescence methods to study protein dynamics in living cells

Traffic. 2000 Oct;1(10):755-62. doi: 10.1034/j.1600-0854.2000.011002.x.


The advent of GFP imaging has led to a revolution in the study of live cell protein dynamics. Ease of access to fluorescently tagged proteins has led to their widespread application and demonstrated the power of studying protein dynamics in living cells. This has spurred development of next generation approaches enabling not only the visualization of protein movements, but correlation of a protein's dynamics with its changing structural state or ligand binding. Such methods make use of fluorescence resonance energy transfer and dyes that report changes in their environment, and take advantage of new chemistries for site-specific protein labeling.

Publication types

  • Review

MeSH terms

  • Energy Transfer
  • Fluorescence
  • Fluorescent Dyes
  • Green Fluorescent Proteins
  • Luminescent Proteins / genetics
  • Microscopy, Fluorescence
  • Proteins / genetics
  • Proteins / physiology*


  • Fluorescent Dyes
  • Luminescent Proteins
  • Proteins
  • Green Fluorescent Proteins