Biosensor for determination of glucose and sucrose in fruit juices by flow injection analysis

Appl Biochem Biotechnol. 2000 Nov-Dec;89(2-3):171-81. doi: 10.1385/abab:89:2-3:171.

Abstract

Glucose and sucrose were measured with an amperometric method by using the flow injection analysis technique. A carbon paste electrode with a renewable surface containing glucose oxidase, horseradish peroxidase, and ferrocene was used in combination with the soluble enzymes invertase and mutarotase. The effect of invertase, mutarotase, and ascorbic acid on the electrode response was examined. Glucose and sucrose concentrations were determined with < 3% errors. The proposed method for glucose and sucrose measurements was validated in real samples of fruit juices. The results were also compared with those obtained with the ultraviolet method.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Ascorbic Acid / chemistry
  • Beverages / analysis*
  • Biosensing Techniques / instrumentation*
  • Calibration
  • Carbohydrate Epimerases / chemistry
  • Dose-Response Relationship, Drug
  • Electrochemistry / methods*
  • Electrodes
  • Ferrous Compounds / chemistry
  • Glucose / analysis*
  • Glucose Oxidase / chemistry
  • Glycoside Hydrolases / chemistry
  • Horseradish Peroxidase / chemistry
  • Metallocenes
  • Models, Chemical
  • Sucrose / analysis*
  • Time Factors
  • Ultraviolet Rays
  • beta-Fructofuranosidase

Substances

  • Ferrous Compounds
  • Metallocenes
  • Sucrose
  • Glucose Oxidase
  • Horseradish Peroxidase
  • Glycoside Hydrolases
  • beta-Fructofuranosidase
  • Carbohydrate Epimerases
  • aldose 1-epimerase
  • Glucose
  • Ascorbic Acid
  • ferrocene